From: Les Hicks <hicks@ualberta.ca>
  To  : RASMB Bulletin Board <rasmb@alpha.bbri.org>
  Date: Fri, 13 Jul 2001 15:57:12 -0600

Differentiating between folded and unfolded states in sed vel

Hi folks,

    Here's a hypothetical question.  Say you have an approximately 7 kDa
protein that is known from other techniques to undergo reversible folding /
unfolding in phosphate buffer.  If the rate of folding / unfolding is fast
(within the time course of a sed vel run, would it be possible to
differentiate between the two states from the sed vel data?  I would think
all you would see is a slightly broader boundary and that you would not be
able to identify two individual boundaries for the two states.  Any takers
on this question?  In any event, have a good week-end.

Regards,

Les Hicks