From: H. Olin Spivey <ospivey@bmb-fs1.biochem.okstate.edu>
  To  : Dmitry Veprintsev , rasmb@alpha.bbri.org
  Date: Sat, 16 Jun 2001 11:39:27 -0500

Re: centerpiece stuck in the housing

Dear Dmitry,

   I think it could be either or both the housing and centerpiece.  Of
course you can find out with a new centerpiece.

   Desiccation will loosen the centerpiece in time.  Cooling might also,
but I am not sure about that - it can't hurt to try and it will be quicker
than waiting for adequate desiccation.  After an equilibrium run at 20 deg.
C, we have placed the cell in a refrigerator.  After stirring the contents
and recentrifuging at 20 deg, the sample will leak out of the cell unless
the screw ring is retightened.  I think this implies that the centerpiece
shrinks more than the housing, but I could be wrong.

   Good luck,
   Olin
______________________________________________________


At 11:14 AM +0100 6/15/01, Dmitry Veprintsev wrote:
>Dear All,
>I have a problem of an epoxy centerpiece stuck in the housing (and the
>other one is being very tight). Is it housing deformation or centerpiece
>swelling? And what would be the remedy?
>As we run only water-based smaples, swelling shouldnt be a problem.
>thanks,Dima
>==================================================================
>Dr. Dmitry Veprintsev; dbv@mrc-lmb.cam.ac.uk;">mailto:dbv@mrc-lmb.cam.ac.uk;
>http://macx48.mrc-lmb.cam.ac.uk
>MRC Centre for Protein Engineering, Hills Rd., Cambidge, CB2 2QH, England
>fax:+44-(0)1223-402140; ph +44-(0)1223-402027; e-fax/voice mail:
>+44-(0)870-125-9849
>PGP public key is on the homepage.


H. Olin Spivey                       Phone: (405) 744-6192
Dept. Biochem. & Molec. Biology      Fax:   (405) 744-7799
246 NRC                              Email: OSpivey@Biochem.Okstate.Edu
Oklahoma State University
Stillwater, OK 74078-3035