From: Michael Brenowitz <brenowit@aecom.yu.edu>
  To  : Borries Demeler <demeler@bioc09.v19.uthscsa.edu>
  Date: Thu, 14 Jun 2001 15:24:28 -0400 (EDT)

Re: RNAse contamination in AUC CP's

On Thu, 14 Jun 2001, Borries Demeler wrote:

> I would like to know if someone has any experience running RNA in an
> AUC over longer time periods in equil. expts. and what measures one can 
> take to eliminate RNAse contamination in the CP's.
> 
> Any suggestions?

  I used a set of "RNA only" cells that were assembled and disassembed in
our RNA laboratory wearing gloves for all manipulations. The cells were
cleaned with "RNaseZap" from Ambion, RNase free water followed by ethanol.

-Michael

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  Michael Brenowitz                              Department of Biochemistry
    Telephone: (718) 430-3179         Albert Einstein College of Medicine
      FAX: (718) 430-8565                       1300 Morris Park Avenue
        brenowit@aecom.yu.edu                         Bronx, NY 10461
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