From: Arthur Rowe <arthur.rowe@nottingham.ac.uk>
  To  : Brian M. Baker <baker@xtal200.harvard.edu>
  Date: Mon, 14 May 2001 10:38:19 +0000

Re: not so strange solvents

Brian -
-
We keep a little H2O18 around - as the O does not exchange with the protein
O, its potentially useful for the well known way of tacking solutes with
mixed chemical species present (bound deteregent, glyco, whatever) where
there is a vbar problem.
However - finding a need which justify the expense ($US hundreds per g) is
hard, and I dread to think what D2O18 costs - as I recall, last time I
looked, it was well into $USK/g !
Our supplier in those days was BOC Ltd, but I can see now sign on their web
site (www.boc.com) that they still do these products. They might however
know who does. Alternatively, try a public utility in the nuclear power
sector - in the days when I was doing zonal runs in H2O-D2O gradients, the
cheapest way to get D2O was from Atomic Energy Authority at Winfrith, UK.
-
Arthur

--
*******************************************************
Arthur J Rowe
Professor of Biomolecular Technology
NCMH Business Centre
University of Nottingham
School of Biosciences
Sutton Bonington
Leicestershire LE12 5RD   UK

Tel:        +44 (0)115 951 6156
             +44 (0)116 271 4502
Fax:        +44 (0)115 951 6157
email:      arthur.rowe@nottingham.ac.uk
             arthur.rowe@connectfree.co.uk (home)
Web:        www.nottingham.ac.uk/ncmh/business
*******************************************************


> From: "Brian M. Baker" <baker@xtal200.harvard.edu>
> Date: Fri, 11 May 2001 15:36:13 -0400
> To: "RASMB List" <rasmb@alpha.bbri.org>
> Subject: not so strange solvents
> 
> On the subject of solvents, where do people get D2O18 from - and more
> importantly, what's it cost?
> 
> -Brian
> 
> ============================================
> Brian M. Baker
> Department of Molecular and Cellular Biology
> Harvard University
> baker@crystal.harvard.edu
> (617) 496-6074
> http://baker.mcb.harvard.edu
> 
>