From: Michael Bovee <mbovee@zoo.uvm.edu>
  To  : rasmb@alpha.bbri.org
  Date: Tue, 13 Mar 2001 13:40:17 -0500

SedEq question

Hello all,
I'm uncertain how to interpret some recent equilibrium experiments, 
since I don't have a lot of experience with this method yet.  Using 
preps of two different protein (complexes) I have gotten acceptable 
preliminary fits of data, but the main thing is that the calculated 
sigma Increases with increasing rotor speed, contrary to what I'm 
reading in the Beckman books and other sources.  (For instance, if 
polydispersity was an issue, I should see a decrease in apparent MW 
at higher speeds)

Maybe I'm getting some kind of non-specific aggregation at higher speeds?

I suspect I haven't given enough details to clarify what could be 
going on, but I'm just looking for some leads on how to continue the 
analysis.  At this point my main purpose is just to obtain a reliable 
weight average MW.

Thanks
-- 
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Michael L. Bovee, Ph.D.
Postdoctoral Fellow
University of Vermont
Department of Biochemistry
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email: mbovee@zoo.uvm.edu
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