From: Henryk Eisenberg <Henryk.Eisenberg@weizmann.ac.il>
  To  : Helmut Coelfen <Helmut.Coelfen@MPIKG-GOLM.MPG.DE>
  Date: Thu, 8 Mar 2001 13:06:46 +0300

Re: Estimate of the density of hydration water

>Hello out there,
>
>for a principal investigation, I'm interested in the structuration of
water by
>polymers expressed in a density increase. We find huge differences in the
>overall solution density when certain synthetic polyelectrolytes are added;
>far
>higher than expected from the buoyancy increment of the polymer. From some
>of the
>last AUC conference talks I think to remember that it was observed that
>hydration
>water of proteins has a higher density than bulk water - probably an effect
>of its
>structuration in the contact area to the protein. Does anyone of you have a
>citeable
>Reference about this effect ?
>
>Thanks a lot in advance for any help you can give.
>
>Best wishes
>
>Helmut Cölfen
>
>

DEAR HELMUT;		MARCH 8, 2001

I SUGGEST YOU CHECK SVERGUN ET AL. PROTEIN HYDRATION IN SOLUTION:
EXPERIMENTAL OBSERVATION BY X-RAY AND NEUTRON SCATTERING. PNAS,
95:2267-2272 (1998) AND EBEL ET AL. PROBING PROTEIN-SUGAR INTERACTIONS,
BIOPHYSICAL J. 78:385-393 (2000).
THIS WILL LEAD YOU TO FURTHER EARLIER ARTICLES.
BEST WISHES, HEINI EISENBERG