From: Brian M. Baker <bbaker2@nd.edu>
  To  : Joel Mackay <j.mackay@biochem.usyd.edu.au>
  Date: Fri, 5 Oct 2001 19:48:46 -0500

RE: light scattering

Hi Joel,
You're right, these are great things to have around, although you need to be
careful in your column selection as particles can come off of the column and
cause you headaches. I've looked at interacting systems as a function of
concentration, but only qualitatively. Go to

http://info.med.yale.edu/wmkeck/6_16_98/Lsmemoa.htm

to get some good background on on-line multi-angle light scattering.

Cheers,
-Brian

=========================================
Brian M. Baker
Department of Chemistry and Biochemistry
251 Nieuwland Science Hall
University of Notre Dame
Notre Dame, Indiana 46556

Email:  bbaker2@nd.edu
Office: 219 631 9810
Lab:    219 631 9775
Fax:    219 631 6652


-----Original Message-----
From: Joel Mackay [j.mackay@biochem.usyd.edu.au]">mailto:j.mackay@biochem.usyd.edu.au]
Sent: Friday, October 05, 2001 5:02 PM
To: rasmb@alpha.bbri.org
Subject: light scattering


hi everyone,
Does anyone have a feel for the utility of multi-angle light scattering for
the determination of molecular weights of proteins in solution? I have only
just come across it as two detectors plumbed into the end of an FPLC - a
light scatterer and an instrument for measuring refractive index. The
literature says that it can reveal MW without a shape dependence, although
it looks like it would be non-trivial if there was more than one species
present (that is, it doesn't look like one could use it easily for
determining association constants etc, just MW for a single purified
species). Seems pretty powerful to be able to get a feel for MW in-line for
each signal off your gel filtration run.
The instruments i saw were from Wyatt (miniDawn and Optilab DSP
refractometer).
cheers
joel

*****************************************************************
Dr Joel Mackay
ARC Research Fellow
Department of Biochemistry, G08
University of Sydney,
Sydney, NSW 2006
Australia
ph +61-2-9351-3906
fax +61-2-9351-4726
WWW: http://www.biochem.usyd.edu.au/staff/mackay/
Sydney Protein Group Website:
http://www.biochem.usyd.edu.au/spg/
****************************************************************