From: Alex Kentsis <kentsis@physbio.mssm.edu>
  To  : judithk@vax2.concordia.ca
  Date: Sat, 29 Sep 2001 10:52:33 -0400

Re: Keq from sedimentatin velocity

dear judith,

for measuring assembly thermodynamics using sedimentation velocity,
consult Stafford WFIII. Sedimentation boundary analysis of interacting
systems: Use of the apparent sedimentation coefficient distribution
function. In: Modern analytical ultracentrifugation. Schuster, T. M.
Laue, T. M., eds. Boston: Birkhauser, 1994.


On Thu, 27 Sep 2001 judithk@vax2.concordia.ca wrote:

> Dear RASMBers, I am trying to measure the equilibrium constant for a
> monomer/dimer system using sedimentation velocity. I feel reasonably sure
> of the s20,w values for the monomer and dimer and reasonably sure that
> there are no higher order mers. However, when calculating Keq, what
> protein concentration do I use? Is it the concentration of the solution
> that I loaded? Or is it the concentration at some point in the cell? If
> the later - at what point in the cell do I measure it and when during the
> run? Many thanks.
> 
> Judith Kornblatt
> Dept of Chemistry and Biochemistry
> Concordia University
> 1455 de Maisonneuve Ouest
> Montreal, Qc H3G 1M8
> Tel: 1 514 848 3384   FAX: 1 514 848 2868
> 
> 
>