From: Hensley, C Preston <c_preston_hensley@groton.pfizer.com>
  To  : rasmb@alpha.bbri.org
  Date: Sat, 25 Aug 2001 14:43:31 -0400

Methods calibration study - a call

All,

We are seeking volunteers to participate in an anonymous analysis of the
state of the art in solution interaction studies.
Below is a proposal to analyze a test system (carbonic anyhydrase and a
small molecule ligand) with the hope to,

1- Separately calibrate AUC, ITC and SPR users against each other.  How does
the practice in your lab compare to the rest of the field?
2- Calibrate the three technologies against each other.  How does the
practice in each field hold up against the others?

The specific questions will be,
For ITC - what is the affinity and thermodynamics of ligand binding?
For SPR - what is the affinity and kinetics of ligand binding?
For AUC - what is the assembly state of the protein (or mass of the
monomer), does it change with ligand binding, or is there a ligand binding
promoted conformational change (use your favorite transport method(s))?

The hope is that this exercise will be useful both for individuals (where do
we each have room for improvement?) and for the fields (what's the real
state of the art?).  We will publish the results anonymously (see below).

If you have more than one of these approaches in you lab, we encourage you
to employ them all.  If you have other relevant techniques in you lab (not
listed above), please include this data (we know there are many).

The project is described in more detail in the attachment.  At this point,
we just want names of volunteer labs.  We will contact you soon and
distribute free reagents and instruction packets.

This is an exciting, and historically significant opportunity.  Separate
calls are going out to members of the ITC and SPR fields.  We already have
many responses.  If this study is successful, there will be more like it
(with different thrusts) in coming years.  We look forward to your
participation and suggestions for future endeavors.

Yours,

Preston
(for the MIRG)

PS:  Please pass this invitation on to other colleagues who may not be on
the RASMB list, who might like to participate.  And, have a look at the ABRF
web site.  This may be a useful society for you. http://www.abrf.org/.  See
also the MIRG, http://www.abrf.org/ABRF/ResearchCommittees/mirg.html

 <<Project2001_Flyer.doc>> 
Preston Hensley
Manager, Protein and Peptide Chemistry
Pfizer Global Research and Development
Pfizer Inc, MS 8118-B3
Eastern Pont Road
Groton, CT 06340
ph: 860-715-2190
fx: 860-441-4734
email: c_preston_hensley@groton.pfizer.com

Gordon Conference, Feb 2002.
Reversible Associations in Molecular and Structural Biology
http://www.bbri.org/rasmb2002/



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