From: Jo Butler <pjgb@mrc-lmb.cam.ac.uk>
  To  : Sandy Go , rasmb@alpha.bbri.org
  Date: Thu, 23 Aug 2001 09:59:03 +0100

Re: Partial Specific Volume of DNA

Dear Sandy,

An historic reference, but still with very carefully measured data for a 
number of physical parameters (including partial specific volumes) of both 
Na DNA and Cs DNA is:
Cohen, G. & Eisenberg, H. (1968). Deoxyribonucleate Solutions: 
Sedimentation in a Density Gradient, Partial Specific Volumes, Density and 
Refractive Index Increments, and Preferential Interactions. Biopolymers 6, 
1077-1100.

Jo

--On Wednesday, August 22, 2001 5:24 pm -0400 Sandy Go 
<sandygo@uhnres.utoronto.ca> wrote:

>
> Hello Everyone,
>
> I would like to know if anybody has done analytical ultracentrifugation
> experiments (sedimentation equilibrium) on DNA samples.  If so, I would
> appreciate it if you could help me with the partial specific volume of
> DNA.  Also, are there any problems that I should anticipate with DNA
> samples?  Thank you.
>
> Sandy Y. Go
> Princess Margaret Hospital
> Toronto, Ontario
> sandygo@uhnres.utoronto.ca
>



P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296