From: Dmitry Veprintsev <dbv@mrc-lmb.cam.ac.uk>
  To  : Bogos Agianian <bogos@vleet.chem.uu.nl>
  Date: Tue, 30 Jan 2001 18:02:12 +0000

Re: A+B Interactions

Hi, Bogos
you can do this in UltraSpin (www.mrc-cpe.cam.ac.uk/ultraspin).
multiple speeds/concentrations will help. 
Using different wavelengths will only be useful if you have different
absorbance spectra (eg, protein-DNA or one of the proteins is labeled). 
Dima
dbv@mrc-lmb.cam.ac.uk
Dr. Dmitry Veprintsev
Centre for Protein Engineering
MRC Hills Rd., Cambridge CB22QH,UK

On Tue, 30 Jan 2001, Bogos Agianian wrote:

> Dear all,
> 
> I would like to investigate an iteresting protein-protein interaction
> system using equilibrium AUC. Based on biochemical data, I anticipate
> that this system could be probably modeled with the 'simple' model
> A + B <-> AB. 
> 
> I would appreciate any suggestions as to which is(are) the best
> program(s) out there to handle such models. I have the impression that
> for such a model even NONLIN could do the job (using a trick which I
> cannot remember).  As I understand it, for a thorough analysis, one
> has to get scans at differend wavelengths (of A and B) apart from
> using differend speeds. However, I would like to know whether there are 
> programs that can model data from the mixture (A+B) collected at only 
> one wavelength.
> 
> Thanks in advance,
> 
> Bogos
> 
> 
> 
>   
> 
> 
> ------------------------------------------------------------------------------
> Bogos Agianian                                
> 
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