From: E.braswell <BRASS@UConnVM.UConn.Edu>
  To  : smorris@medusa.bioc.aecom.yu.edu
  Date: Fri, 27 Jul 2001 14:12:16 EDT

small molecules

Ms Morris,  Yes, Philo is completely right (as usual). My answer assumed
that you wanted to determine not just MWT but also stoichiometry and equil.
constants of a possibly associating system.  Then you MUST have more curvature
in your data whether it is absorption or interference. With this in mind,
my comments were appropriate.

Emory H. Braswell
Professor of Biophysics and Head,
National Analytical Ultracentrifugation Facility
U-149
University of Connecticut
STORRS, CT 06269-3149
TEL 860 486 5032
FAX         5005
EMORY.BRASWELL@UCONN.EDU