From: Arthur Rowe IMAP <arthur.rowe@nottingham.ac.uk>
  To  : Stephanie Morris <smorris@medusa.bioc.aecom.yu.edu>
  Date: Fri, 27 Jul 2001 11:22:36 +0000

Re: small peptides

Stephanie -

You can get a bigger signal to work with for equilibrium studies by upping
the concentration of your solute (peptide), if that is possible. I recall
once checking out the MW of raffinose this way, and got the answer spot-on -
even a shot at a virial coefficient. Even without going as high in c as we
did there (around 40 mg/ml, if I recall right), a decent MW estimate should
be attainable.

Regards

Arthur
--
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Arthur J Rowe
Professor of Biomolecular Technology
NCMH Business Centre
University of Nottingham
School of Biosciences
Sutton Bonington
Leicestershire LE12 5RD   UK

Tel:        +44 (0)115 951 6156
             +44 (0)116 271 4502
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email:      arthur.rowe@nottingham.ac.uk
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Web:        www.nottingham.ac.uk/ncmh/business
*******************************************************


> From: Stephanie Morris <smorris@medusa.bioc.aecom.yu.edu>
> Date: Thu, 26 Jul 2001 13:09:15 -0400 (EDT)
> To: rasmb@alpha.bbri.org
> Subject: small peptides
> 
> Dear RASMBers,
> 
> I was wondering if anyone has performed sedimentation velocity or
> sedimentation equilibrium experiments on small peptides?  Recently, I
> performed a sed velocity experiment with a peptide that is about 2,000Da
> at a speed of 55,000rpm.  The boundaries were very broad and difficult to
> analyze using programs such as DCDT+ and SVEDBERG. Does anyone have any
> suggestions for running sed. velocity experiments using very small
> peptides and for analyzing this data?  I'm also interested in performing
> sed. equilibrium experiments with this peptide. Are there any quirks or
> helpful tips for running these kinds of experiments?  Any suggestions
> would be greatly appreciated.
> 
> Thank you very much,
> 
> Stephanie
> 
> Stephanie Morris
> Albert Einstein College of Medicine
> 1300 Morris Park Ave.
> Bronx, NY 10461
> smorris@aecom.yu.edu
> 
>