From: Stephanie Morris <smorris@medusa.bioc.aecom.yu.edu>
  To  : rasmb@alpha.bbri.org
  Date: Thu, 26 Jul 2001 13:09:15 -0400 (EDT)

small peptides

Dear RASMBers,

I was wondering if anyone has performed sedimentation velocity or
sedimentation equilibrium experiments on small peptides?  Recently, I
performed a sed velocity experiment with a peptide that is about 2,000Da
at a speed of 55,000rpm.  The boundaries were very broad and difficult to
analyze using programs such as DCDT+ and SVEDBERG. Does anyone have any
suggestions for running sed. velocity experiments using very small
peptides and for analyzing this data?  I'm also interested in performing
sed. equilibrium experiments with this peptide. Are there any quirks or
helpful tips for running these kinds of experiments?  Any suggestions
would be greatly appreciated.

Thank you very much,

Stephanie

Stephanie Morris
Albert Einstein College of Medicine
1300 Morris Park Ave.
Bronx, NY 10461
smorris@aecom.yu.edu