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From: Bogos Agianian <bogos@octopus.chem.uu.nl>
To : rasmb <RASMB@BBRI.ORG>
Date: Wed, 5 Jul 2000 15:17:29 +0200 (MZT)
Compatible B values
Dear all,
I wonder how comparable AUC derived virial coefficients (e.g, from
NONLIN) are to B22 values obtained from other methods (e.g. static
light scattering). This is of particular importance to us, because
such B22 values could, among other things, be used to predict protein
crystal growth.
Related to this, is the issue of the unit and sign definitions used
for Bs. As far as I've seen, the standard unit from non AUC methods
is mol.ml.g-2, whereas NONLIN reports B*Mmonomer in units of mm-1, if
I'm not mistaken. Negative or positive sign have also been given
conflicting meanings throughout the literature.
I would appreciate if somedody with much greater exprertise than I
have could enlighten me on these issue.
Thanks in advance,
Bogos Agianian
PS: "I take this opportunity to thank all who have spent precious time
to reply to previous questions I posted on this list."
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Bogos Agianian
Dept. of Crystal & Structural Chemistry | TEL (Office): +31 30 253 2868
Utrecht University/Bijvoet Center | TEL (Lab) : +31 30 253 9182
Padualaan 8, 3584 CH Utrecht | FAX : +31 30 253 3940
The Netherlands | http://www.crystal.chem.uu.nl/~bogos
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