From: E.braswell <BRASS@UCONNVM.UCONN.EDU>
  To  : rasmb@alpha.bbri.org
  Date: Fri, 28 Apr 00 14:41:04 EDT

PEG

No, John, Not using synthetic boundary--I mean if you are looking at
several PEGylated species of the same protein by sed vel you can estimate
the amount of protein in each one by absorbance (if you know the extinction).
Then by the number of fringes in each peak and by knowing dn/dc of the protein
and the PEG, you can calculate the degree of PEGylation of the protein.
         Thanks so much for your answer John--and for the great answers by
everyone!                                      Emory

Emory H. Braswell
Professor of Biophysics and Head,
National Analytical Ultracentrifugation Facility
U-149
University of Connecticut
STORRS, CT 06269-3149
TEL 860 486 5032
FAX         5005
E.BRASWELL@UCONN.EDU