From: E.braswell <BRASS@UCONNVM.UCONN.EDU>
  To  : rasmb@alpha.bbri.org
  Date: Thu, 27 Apr 00 20:52:19 EDT

incompetency

John, I don't know much about folding, or its dynamics, but can't molecules
be locked in a number of nearly similar configurations or be "misfolded"
(as I've heard the term used by people who produce proteins by recombinant
methods)?   This was what I was thinking of, and a number of such misfolded
species could conceivably cause a range of lnKs.
It would be nice if we could produce a graph of the distribution of lnKs for
a given species in solution in order to characterize these.
I have contributed my 2 cents more on this topic in the last 2 days than I
usually do in a year--so please bear with me and give us one more John!
and any others please.
  Next question, does anyone know if glycosylation can do most of the nice
things that PEGylation does for pharmaceuticals?  Incidentally, I am
ignorant of the literature, but I have lately tried estimating degrees
of PEGylation and glycosylation, by measuring sed velocity with both
interference and absorption at 280.  I know of some gotchas but there are
probably more.  Any references anybody?
                                           Regards, Emory

Emory H. Braswell
Professor of Biophysics and Head,
National Analytical Ultracentrifugation Facility
U-149
University of Connecticut
STORRS, CT 06269-3149
TEL 860 486 5032
FAX         5005
E.BRASWELL@UCONN.EDU