From: Joel Mackay <j.mackay@biochem.usyd.edu.au>
  To  : rasmb@alpha.bbri.org
  Date: Wed, 11 Oct 2000 17:12:46 +1000

RE: negative XL-A data - summary

hi all,
Thanks for the replies to my queries. The general consensus is that 
reducing agents are a bit of a pain, and one should avoid them where 
possible, or prepare them as freshly as possible, keep things cool if 
possible and hope for the best. TCEP and TBP (Tri-n-butylphosphine) have 
been touted as alternatives, but apparently TCEP at least has met with 
mixed results in terms of being a non-absorber in the UV. It is certainly 
true that it is not compatible with all buffers and i suspect it  probably 
works best (ie closer to the advertising claims) at lower pHs.

It appears that my wavelength jumping problem may be a firmware problem 
that can be addressed by the service people.

cheers,
joel

*****************************************************************
Dr Joel Mackay
ARC Research Fellow
Department of Biochemistry
University of Sydney,
Sydney, NSW 2006
Australia
ph +61-2-9351-3906
fax +61-2-9351-4726
WWW: http://www.biochem.usyd.edu.au/staff/mackay/
Sydney Protein Group Website:
http://www.biochem.usyd.edu.au/spg/
****************************************************************