From: Vincent Shier <vshier@babylon.chem.psu.edu>
  To  : rasmb@alpha.bbri.org
  Date: Mon,  9 Oct 2000 22:22:42 -0400

Re: negative XL-A data

Howdy,
   I agree with Borries on this one, for the most part.  DTT and/or BME are horrible with absorbance optics, unless you are working with rather high concentrations of proteins or wavelengths above 250nm.  Otherwise, your best bet is to use interference optics.  Where I disagree with Borries is that TCEP is better.  Molecular Probes markets it as a none 
absorbant reducing agent.  In our hands, this has not been the case!  It is slightly less absorbant than BME or DTT, but it still gives you the same problems in AU experiments.  So, stick with interference if you can.  


      Vince



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Vincent K. Shier
Department of Chemistry
Penn State University
152 Davey Laboratory, Box 73
University Park, PA 16802
(814) 865-9508
vks3@psu.edu
     or
vshier@chem.psu.edu

http://www.personal.psu.edu/vks3
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