From: Brian M. Baker <baker@xtal200.harvard.edu>
  To  : rasmb@alpha.bbri.org <rasmb@alpha.bbri.org>
  Date: Wed, 13 Sep 2000 19:45:10 -0400

help interpreting light scattering?

Greetings RASMBers,
I've come across a situation where I need to interpret some light
scattering results. Basically, I have centrifuge (equilibrium and
velocity) data that argues against oligomerization of a complex; these
results conflict with a literature report that claims, using
quasi-elastic light scattering, to show oligomerization of a very
similar complex. I've done a wee-little bit of dynamic light
scattering, but not enough to critically examine this manuscript. I
don't put a lot of stock in the paper. My problem stems from the
analysis of solutions which contain at a minimum 3 species (A, B, AB)
as bimodal. I'm hoping that somebody might be able to help me out,
hopefully somebody who really knows this stuff can look at this and
make an informed opinion in only a few minutes.

The paper of interest is Nature, vol. 387, 617-620, 1997. I can supply
the pdf if necessary. Any takers? This is might be an opportunity to
show that AUC is a standard to which other methods can be held...

Thanks,
-Brian

============================================
Brian M. Baker
Department of Molecular and Cellular Biology
Harvard University
baker@crystal.harvard.edu
(617) 496-6074