From: Emmanuelle <delagoutte@morel.uoregon.edu>
  To  : RASMB@BBRI.ORG
  Date: Wed, 21 Jun 2000 13:56:52 -0700

Dear RASMB Members:

	I am forwarding a request from someone in our institute in order to
get an answer from experts.
	Thank you for your help.

		Emmanuelle Delagoutte.


Bruce Birrell wrote:


The protein we work with in the Griffith Lab, phospholipase C (molecular
weight 35,000), appears to have a binding site for one phospholipid
 molecule (molecular weight 450), and we are wondering if it is possible to
measure a dissociation constant for this interaction using the analytical
ultracentrifuge.  We have no idea if this is a reasonable way to
obtain this information, but we were wondering if you know anyone in the
Analytical Ultracentrifugation community who might be knowledgeable about such
things.  If so, we could contact them, and get a better idea if this is
something we should try.

Thanks a lot,

 Bruce

Emmanuelle Delagoutte
Institute of Molecular Biology
University of Oregon
Eugene, OR, 97403
Tel (work) : 541 346 5147
Tel/Fax (home): 541 342 6157
e-mail : delagoutte@molbio.uoregon.edu