From: Arthur Rowe <Arthur.Rowe@nottingham.ac.uk>
  To  : RASMB <RASMB@BBRI.Harvard.Edu>
  Date: Tue, 13 Apr 1999 16:19:29 GMT0BST

re: Analysis of ultracentrifugation data/further

Whilst Henryk Eisenberg's proposal is (obviously) totally correct in principle, it is 
necessary to bear in mind that the dialysis to equilibrium of a solution in 20% 
glycerol may take quite some time - possibly even a day or more with stirring.

And the protein concentration will surely change as a result of osmotic effects, so 
the protein concentration would need to be measured AFTER dialysis (always good 
practice, of course).  I suppose that if the extinction coefficient is well defined, then 
it will be OK to use it to estimate the concentration in the 20% glycerol (cannot 
think of any good reason why not, but have never actually done it !)

Arthur Rowe
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Professor Arthur J Rowe
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University of Nottingham
School of Biological Sciences
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