From: John Correia <jcorreia@biochem.umsmed.edu>
  To  : rasmb@alpha.bbri.org
  Date: Fri, 05 Mar 1999 09:48:57 -0600

Re: Sednterp updates?

Arturo,

   John Philo is correct, new data is sparse.   We have made density
measurements on Pipes & Mes buffers at various concentrations up to 100 mM
and temperatures from 4 to 40oC on a new Anton Paar density meter.  Its
good to 5 decimal places, works with a peltier cell over the range of 0 to
80oC, & I highly recommend it.   [ Only $18K ]  Measurements are very
reproducible and fast.  We now typically do measurements on all buffers. 
Viscosity is another story - its slow and tedious and difficult.

   We will eventually release the Pipes & Mes data, but there is a
problem:  Sedenterp assumes temp corrections should parrallel that of
water.  We have found deviations from water behavior, and thus we need a
way to correct for both temperature and buffer concentrations.  I have
only briefly discussed this with Tom Laue, but maybe changes can be made
Sednterp that allow these changes to be introduced easily.  I'll see John
& Tom at the workshop & we'll try to map out a plan.







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     Arturo J. Morales    (RPI '94)             art@scripps.edu 
   Department of Biology - Massachusetts Institute of Technology &
  Skaggs Institute for Chemical Biology - Scripps Research Institute
                   http://schimmel.scripps.edu/~art

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 Dr. John J. "Jack" Correia
 Department of Biochemistry
 University of Mississippi Medical Center
 2500 North State Street
 Jackson, MS  39216
 (601) 984-1522                                 
 fax (601) 984-1501                             
 new email address: jcorreia@biochem.umsmed.edu     
 homepage: http://fiona.umsmed.edu/~biochem/correia.html  
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