From: Andrew Leech <A.Leech@uea.ac.uk>
  To  : RASMB@BBRI.ORG
  Date: Fri, 17 Dec 1999 15:47:58 +0000 (GMT)

Anaerobic use and C-filled Epon

I would be interested in hints and tips about ultracentrifugation
of oxygen sensitive (protein) samples. I am worried that residual
O2 might stick to the surface of the charcoal-filled Epon cells.
My current idea is to store the assembled cells in a glove box
for some period then fill them, then transfer out to the machine
(which is some distance away).

Am I being paranoid, or are there techniques to minimise oxygen
contamination during cell filling and run setups? Any advice would
be greatly appreciated.

Andrew

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Dr Andrew Leech                  *Tel +44 (0) 1603 592758
Scientific Officer               *Fax +44 (0) 1603 592250
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