From: Elizabeth Reisinger aka Libby <libby@groucho.med.jhmi.edu>
  To  : RASMB@bbri.org
  Date: Fri, 12 Nov 1999 16:14:57 -0500 (EST)

s coeff and shape

Hello RASMBers,

We are working on a protein that we believe is asymmetric.  I'm trying to
get sedimentation velocity data to hopefully support this hypothesis.  I
do NOT need an exact, definitive shape, all I am interested in is knowing
that our proposed (asymmetric) model is one way of interpreting the
sedimentation coefficient.

I know the following:

1.  protein is a single species by sed. eq.
2.  s shows concentration dependence (2.5-4 S) by vanHolde-Weischet
analysis

My questions:  

What analysis method(s) is the best for my problem ??  

If s is concentration dependent, may I take an average s from a g(s)
analysis and make crude shape predications based on that ??

Thanks in advance for your advice,

Libby