From: Arturo Morales <art@scripps.edu>
  To  : rasmb@bbri.org
  Date: Sat, 6 Nov 1999 20:39:47 -0800 (PST)

Simulating Eq curves

Hi there,

We have a RNA:protein system in which we are calculating a MW  ~75Kd.
This could either represent a 2:1 RNA:protein or a 1:2 complex. (since the
RNA and the protein monomer are both ~25Kd.  We can make an argument for a
1:2 complex since the 260:280 ratio appears to be closer to what we would
expect in that type of complex (it is not quite 2:1 as we would expect for
a complex with more RNA than protein.  We would like to model the expected
eq curves for both possibilites using theoretical data and compare it with
our actual data.  Altough we have a bunch of different software titles
that can deal with velocity data, we only have the beckman stuff for
equilibrium... Any suggestions? :)

Thanks!

Art

----------------------------------------------------------------------
Arturo J. Morales    (RPI '94) |  art@scripps.edu
Department of Biology          | Massachusetts Institute of Technology
  Skaggs Institute for Chemical Biology - Scripps Research Institute
                  http://minihelix.mit.edu/art