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  From: Karen Gibson Fleming <kgf@csb.YALE.EDU>
  To  :
  Date: Thu, 23 Apr 1998 10:37:27 -0400

standard proteins that dimerize

Hello all,

An investigator here at Yale has run across a weak monomer:dimer
interaction that they have characterized using the mini-dawn light
scattering machine. They believe from their
analysis of the data that it has a Keq of about 5e4.

They would like to look at a few standard proteins that might have
slightly stronger Keq for dimer formation, in order to make sure that
the method by which they derived their Keq is valid.

This work would have to be done at room temperature. I am aware of
the monomer-dimer equlibrium of alpha-chymotrypsin at pH. 4.0. Does 
anyone have any other suggestions for candidate proteins that this
person could purchase for a reasonable price and use as a standard??
Specific literature references would be helpful.



Karen Gibson Fleming, PhD
Yale University, Department of Molecular Biophysics & Biochemistry
266 Whitney Avenue, PO Box 208114, New Haven, CT  06520-8114
Voice (203) 432-5174     Fax (203) 432-5175
If you are not part of the solution, then you are part of the

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