From: Houphouet Hy Yarabe <hyarabe@unix1.sncc.lsu.edu>
  To  : Jeffry C. Nichols <jcn@bioc.rice.edu>
  Date: Thu, 16 Apr 1998 13:21:16 -0500 (CDT)

Re: v-bar calculations

On Tue, 14 Apr 1998, Jeffry C. Nichols wrote:

> A coworker here mentioned that she heard at a recent
> conference in Galveston, TX a program for calculating
> v-bar in the presence of denaturant, and that the calculated
> results were in close agreement to measured values.
> 
> Does anyone know of this program or the specific equations
> for calculating v-bar?
> 
> Thanks for the help.
> 
> 
> Jeff Nichols, PhD
> Rice University
> Biochemistry Dept.
> Houston, TX  77005
> 
I did use the following equation to calculate the partial specific volume 
of my detergents:
		1/density = v-bar + W1d(1/density)/dw1.  This relation
can be found in any p-chem book.  It is easy to derived it from partial
molar quantities, followed by a systematic transformation in term of
partial specific quantities.  The key is to measure the density of the
detergents at different concentrations (if you got a densitymeter).
Then,plot 1/density as a function of W1 (weight fraction).
The linear portion of the graph will provide you with a slope and an
y-intercept.  The y-intercept would be the partial specific volume.  If
you have more question about this, let me know.

Best regards,

Yarabe> >