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From: Dr A.J. Rowe <ajr@leicester.ac.uk>
To : David Scott <djs17@york.ac.uk>
Date: Mon, 14 Dec 1998 15:41:44 +0000 (GMT)
Re: exctinction coefficients at 230 nm
Hi Dave !
The obvious warning one has to give in this matter concerns the Rayleigh
scattering component, which will contribute unequally at the two wavelengths.
However - one of the joys of the AUC is that the small amounts of heavy
aggregate which plague regular spectroscopy are pretty much removed from the
opitcal path, thanks to g-force.
Given the limited precision which inevitably dogs Kd determinations, I doubt if
you have anything significant to worry about from this cause, at least. We
certainly find we can do Kd*s in the far uv and be reasonably happy with the
outcomes.
All best
Arthur
***************************************************
Arthur J Rowe
Professor of Biomolecular Technology
University of Nottingham
The NCMH Business Centre
School of Biological Sciences
Sutton Bonington
Leicestershire LE12 5RD, UK:
Tel/voicemail : +44 (0)115 951 6156
Tel/Fax : +44 (0)115 951 6157
arthur.rowe@nottingham.ac.uk}
or: sczarr@szn1.nott.ac.uk
***************************************************
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