From: Richard.PedrettiAllen@octel.com
  To  : sjlessey@concentric.net
  Date: Thu, 31 Jul 1997 11:20:55 -0700

RE: Denaturing Protein with Gua-HCl

My advice is an obvious oversimplification of a relatively trivial
question but I'm not about to go into protracted dissertations about
protein decomposition or quantifications of reaction times in a series
of graded temperature classes with a complete stranger!  My goodness!
What would people think?!  

Besides, this a distinguished member of the Department of Biochemical &
Molecular Biology of Oklahoma State University!  Why doesn't he go to
the library and look it up?

Mind the gap.

Cheers, Richard

>----------
>From: 	ANDREWS, CHRIS[SMTP:ANDREWSC@pab281a.ssd.loral.com]
>Sent: 	Thursday, July 31, 1997 11:36 AM
>To: 	Davis, Michael via voicemail; 72650.3277; ANDREWS.CHRIS; bill.thomson;
>catfishzz; dbrenner; dky; droo.thomson; dsteinberg; gluthr; grenier2;
>kamradtJ; ospivey; paulb; paulg; RASMB; Richard.PedrettiAllen; russell;
>sjlessey; wsand
>Subject: 	RE: Denaturing Protein with Gua-HCl
>
>
>Well ,  I must say that I agree with Richard's assessment.
>After all , you can't make butter with a toothpick , you know what
>I'm sayin'?
> ----------
>From: Richard.PedrettiAllen
>To: Michael.Davis; 72650.3277; ANDREWS.CHRIS; bill.thomson; catfishzz; 
>dbrenner; dky; droo.thomson; gluthr; grenier2; kamradtJ; paulb; paulg; 
>richard.pedrettiallen; russell; sjlessey; wsand; dsteinberg; ospivey; RASMB
>Subject: RE: Denaturing Protein with Gua-HCl
>Date: Thursday, July 31, 1997 10:14AM
>
>Not really since you don't have much proteolytic activity in 6M
>guanidinium at any temperature, since it is such a strong
>chaotropic agent.
>
>Cheers, Richard
>>
>>Dear RASMBers,
>>
>>   When testing for protein subunit molec. weights using 6 M
>>guanidinium-HCl, is it wise to add the protein sample to nearly boiling
>>guanidinium-HCl to avoid potential proteolytic activities as is done with
>>SDS denaturation?
>>
>>   Thanks,
>>   Olin
>>
>>H. Olin Spivey                    Phone: (405) 744-6192 or -6189
>>Dept of Biochem. & Molec. Biol.     Fax: (405) 744-7799
>>246 NRC
>>Oklahoma State University
>>Stillwater, OK 74078-0454        E-Mail: Ospivey@BIOCHEM.OKSTATE.EDU
>>
>>
>>(See attached file: rfc822.txt)
>>
>