From: H. Olin Spivey <ospivey@bmb-fs1.biochem.okstate.edu>
  To  : RASMB@BBRI.HARVARD.EDU
  Date: Wed, 30 Jul 1997 10:56:28 -0600

Denaturing Protein with Gua-HCl

Dear RASMBers,

   When testing for protein subunit molec. weights using 6 M
guanidinium-HCl, is it wise to add the protein sample to nearly boiling
guanidinium-HCl to avoid potential proteolytic activities as is done with
SDS denaturation?

   Thanks,
   Olin

H. Olin Spivey                    Phone: (405) 744-6192 or -6189
Dept of Biochem. & Molec. Biol.     Fax: (405) 744-7799
246 NRC
Oklahoma State University
Stillwater, OK 74078-0454        E-Mail: Ospivey@BIOCHEM.OKSTATE.EDU