From: Tom Laue <tom.laue@unh.edu>
  To  : rasmb@bbri.harvard.edu
  Date: Fri, 18 Apr 1997 07:19:39 -0400

Re: Experiments in glycerol

Steve,
We worked with an interacting system in 40% glycerol. The solution is like
honey, so there is no hope for velocity work. Your only hope is to use
short solution columns and wait for equilibrium... forever. We found with
0.75 mm columns that 24 hrs or more were required (and we were at 20 C). Be
sure to use a program like Match (at the RASMB site) to determine
equilibrium- you can be fooled easily by subtracting scans.

Arthur is right about vbar, and I don't know of anyone who has done
systematic work on that (although Timasheff may have). Let me know if you
find anyone who has any idea about the general trend for vbar in glycerol.

Often the high dipole moment of DNA-binding proteins causes aggregation.
This is usually reported as 'instability' by the persons working with the
protein. I have found that high salt sometimes makes these balky systems
behave better.

Good luck!
Tom
Tom Laue
Biochemistry and Molecular Biology
University of New Hampshire
Durham, NH 03824
Ph:  603-862-2459
FAX: 603-862-4013