From: Philo, John <jphilo@amgen.com>
  To  : 'Byron, Olwyn' <o.byron@bio.gla.ac.uk>
  Date: Thu, 18 Dec 1997 08:23:39 -0800

RE: PEG

There are vbar data for shorter PEGs (200, 400, 600, 1000) in Arakawa &
Timasheff (1985) Biochemistry 24, 6756-6762.

John

> ----------
> From: 	o.byron@bio.gla.ac.uk[SMTP:o.byron@bio.gla.ac.uk]
> Sent: 	Thursday, December 18, 1997 7:05 AM
> To: 	rasmb@bbri.harvard.edu
> Subject: 	PEG
> 
> 
> Hello RASMBers
> 
> I've got two quick questions to ask concerning polyethylene glycol
> (PEG).
> 
> We're trying to characterise a protein-PEG system and are having real
> trouble measuring a vbar for the conjugated molecule. However, we know
> the
> mass fraction of PEG and so conceivably we could estimate a vbar if we
> knew
> the vbar of the PEG we're using.
> 
> So, does anyone have any vbar data for PEG?
> In particular we're after vbar for PEG 5,000, 10,000, 20,000 and
> 40,000 (Da)
> (I don't know that vbar for these will necessarily be comparable).
> 
> The second question concerns labelling PEG: does anyone know of an
> easy way
> to covalently (or non-covalently) label PEG (so that we can try AUC
> studies
> using absorbance optics)?
> 
> Thanks in advance for your responses to these questions.
> All the very best for Christmas and the New Year-
> 
> Olwyn Byron.
> 
> 
> _____________________________________________________________________
> Olwyn Byron
> Division of Infection and Immunity
> Institute of Biomedical and Life Sciences
> Joseph Black Building, University of Glasgow
> Glasgow, G12 8QQ, Scotland.
> 
> tel: +44 (0)141 330 3752  email: o.byron@bio.gla.ac.uk
> fax: +44 (0)141 330 4600  web:   www.gla.ac.uk/Acad/IBLS/II/ob/ob.htm
> _____________________________________________________________________
> 
>