From: Olwyn Byron <o.byron@bio.gla.ac.uk>
  To  : rasmb@bbri.harvard.edu
  Date: Thu, 18 Dec 1997 16:05:37 +0100

PEG

Hello RASMBers

I've got two quick questions to ask concerning polyethylene glycol (PEG).

We're trying to characterise a protein-PEG system and are having real
trouble measuring a vbar for the conjugated molecule. However, we know the
mass fraction of PEG and so conceivably we could estimate a vbar if we knew
the vbar of the PEG we're using.

So, does anyone have any vbar data for PEG?
In particular we're after vbar for PEG 5,000, 10,000, 20,000 and 40,000 (Da)
(I don't know that vbar for these will necessarily be comparable).

The second question concerns labelling PEG: does anyone know of an easy way
to covalently (or non-covalently) label PEG (so that we can try AUC studies
using absorbance optics)?

Thanks in advance for your responses to these questions.
All the very best for Christmas and the New Year-

Olwyn Byron.


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Olwyn Byron
Division of Infection and Immunity
Institute of Biomedical and Life Sciences
Joseph Black Building, University of Glasgow
Glasgow, G12 8QQ, Scotland.

tel: +44 (0)141 330 3752  email: o.byron@bio.gla.ac.uk
fax: +44 (0)141 330 4600  web:   www.gla.ac.uk/Acad/IBLS/II/ob/ob.htm
_____________________________________________________________________