From: Lisa Joss <lisa.joss@genetics.utah.edu>
  To  : Marcus Wagner <marcus@Nemesis.chemie.uni-stuttgart.de>
  Date: Thu, 20 Nov 1997 08:52:16 -0700

Re: cell leakage

Marcus,

In my experience, leakage with skinny centerpieces can be a major problem if the
faces of the centerpiece are not lapped properly - though this is not an easy
problem to fix!!! Try coating the faces with a thin layer of vacuum grease. If
all else fails maybe you could try tightening the screw ring a little more.

Good Luck

Lisa



Marcus Wagner wrote:

> I got problems with a 3 mm double-sector centerpiece (Epon, charcoal-filled).
> At 20000 rpm or more cell leakage occures. Does anybody know how to prevent
> this? I tourqued the screw ring to 120 inch-pounds as usual. With the 12 mm
> double-sector centerpieces I got no problems up to 42000 rpm.
>
> MARCUS WAGNER
>
> you may also reply to
> marcus@medusa.chemie.uni-stuttgart.de