From: Jo Butler <pjgb@mrc-lmb.cam.ac.uk>
  To  : parker@topaz.microbio.uab.edu
  Date: Thu, 13 Nov 97 11:26:37 +0000

Re: Cleaning XL cells and windows

>From: "Matthew Parker" <parker@topaz.microbio.uab.edu>
>Date: Wed, 12 Nov 1997 09:50:12 -0600
>To: rasmb@bbri.harvard.edu
>Subject: Cleaning XL cells and windows
>
>Hi! I was wondering if I could get people's opinions about the most
thorough
>way to clean the cells and windows used in the XL-A. We soak them in 0.1
% SDS,
>but I've heard that something like 0.1 N HCl might work better for
getting rid
>of any proteins which might be stuck.
>
>	Thanks,
>
>	Matthew Parker


We have used a rinse with 0.1% SDS, followed by water and ethanol, for
many
years, on both the centrepieces and windows.  We have no problem with
residual
adsorption on the windows and also have been able to run RNA in the cells
without degradation, suggesting that there is little, if any, carry over
from
other samples.

Jo

-----------------------------
Jo Butler, 
MRC LMB,
Cambridge, UK.
Tel:+44 1223 248011
FAX:+44 1223 213556
Email: pjgb@mrc-lmb.cam.ac.uk
-----------------------------