From: CS Raman <raman@hemebase.bio.uci.edu>
  To  : Richard.PedrettiAllen@octel.com
  Date: Thu, 31 Jul 1997 12:12:59 -0700 (PDT)

Re: Denaturing Protein with Gua-HCl

{*} Not really since you don't have much proteolytic activity in 6M
{*} guanidinium at any temperature, since it is such a strong 
{*} chaotropic agent.
{*} 

One has to approach this with caution.  It depends on the type of 
protease you are using.  There are a few proteases that are fully
active in 6 M Gdn.HCl or 9 M Urea.  If using the conventional 
serine proteases then Richards' statement would be o.k.

Cheers
-raman
___________________________________________________________________
C.S.Raman                    Tel: (714) 824-4322
University of California     Fax: (714) 824-8540 
Dept. MB & B                 email: raman@indigo1.bio.uci.edu
3205 Bio Sci II
Irvine, CA 92697-3900
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