From: Matthew Parker <parker@topaz.microbio.uab.edu>
  To  : RASMB@bbri.harvard.edu, prevelig@topaz.microbio.uab.edu
  Date: Thu, 3 Oct 1996 14:39:27 -0500

High offset (E) values when fitting eq. data

	I am using the Microcal Origin software to fit sedimentation
equilibrium data obtained with an XLA. I am finding that I need to float the
offset ("E") values in order to get reasonable fits, and that some of the E
values are turning out to be very high (0.2 to 0.4 absorbance units).
Unfortunately, I didn't do an overspeeding to obtain the true E values (I'm new
at this), but I really doubt that they are actually that high.

	Does anybody have any comments or suggestions? Can I use this data?

	Thanks,

	Matthew Parker
	University of Alabama at Birmingham