From: Walter Stafford <STAFFORD@bbri.harvard.edu>
  To  : rasmb@bbri.harvard.edu
  Date: Wed, 17 Jan 1996 23:32:57 -0500 (EST)

National AUC Workshop Announcement

                       ANALYTICAL ULTRACENTRIFUGATION
                             Theory and Practice

                         WORKSHOP March 19-21, 1996

                                    and

                          Symposium March 22, 1996

              National Analytical Ultracentrifugation Facility
  Biotechnology Center, University of Connecticut, Storrs, Connecticut, USA

   * Interact with national experts in analytical ultracentrifugation.
   * Learn characterization of recombinant produced proteins
   * Work with hardware and software developed on-site at the National
     Analytical Ultracentrifugation Facility.
   * Receive copies of all data analysis for your subsequent use.
   * Receive individual attention to your research interests.
   * See the Beckman XL-A Analytical Centrifuges in Operation
   * See real time interferometry in operation on Model E and Beckman XL-I
     analytical ultracentrifuges.
   * Learn about new specialized centrifuge cells.
   * Receive registration for Analytical Ultracentrifugation symposium to
     follow workshop.

INTENT and DESCRIPTION

Analytical Ultracentrifugation (AU) is a powerful method for the
determination of absolute molecular weights and the study of
self-association and interactions between biological molecules in solution.
The combination of new computerized instrumentation, more rapid experimental
protocols, and powerful data analysis techniques have led to a resurgence of
interest in AU, particularly in biotechnology and pharmaceutical research.

The AU Facility in the Biotechnology Center was established in 1988 by
grants from the National Science Foundation and the Connecticut Department
of Higher Education. Creation of the facility follows the fundamental
contributions of its staff to the development of AU to a wide range of basic
and applied problems for over a quarter century. The staffed facility houses
both Beckman Model E and XL-A analytical ultracentrifuges with their
associated equipment. The facility is available to investigators for basic
research through facility scientists or by collaboration with one of its
principal investigators. Research projects from industry are encouraged.

The Analytical Ultracentrifugation Facility is pleased to offer an AU
workshop designed for professionals with some previous knowledge of AU
including:

   * Industrial Scientists

   * Academic Scientists

   * Research Technicians

   * Students

The workshop will provide a conceptual framework and hands-on experience in
modern computer-assisted data acquisition and data analysis for molecular
weight determination, for study of molecular association-dissociation
reactions and for determination of attendant equilibrium constants, with
applications to proteins, glycoproteins and nucleic acids. One session will
be devoted to development of experimental strategies for designing various
kinds of molecular characterization problems.

Sponsored by the UConn Biotechnology Center, Beckman Instruments, Inc.,
UConn Department of Molecular & Cell Biology, Digital Equipment Corporation,
Genentech, Inc., National Science Foundation, Perkin Elmer, Pfizer, Inc.,
SmithKline Beecham Pharmaceuticals, and others to be announced at the
workshop.

SPECIAL PROGRAM FEATURES:

   * Limited workshop size for maximum interaction

   * Interactive lecture sessions

   * Data analysis software furnished to all participants

The registration fee of $1500 (or $800 for registrants of not-for-profit
organizations) includes all costs of instruction, materials, software,
parking, refreshments, lunches and reception. The fee does not include hotel
accommodations.

Application is necessary. Faxed (203-486-5005) applications are encouraged.
A letter of acceptance will include information on housing, transportation
and an invoice for the registration fee, as appropriate.

For Information on:

   * Course content: call Todd Schuster at 203-486 4333

   * Payments and course logistics: call the Biotechnology Center at
     203-486-5011

   * Fellowships and Applications: call 203-486-5011

   * E-mail: Biotctrl@uconnvm.uconn.edu

Some registration fellowships are available to qualified graduate and
post-doctoral students. Call or send for a fellowship application form.

Refunds and Cancellations The registration fee is fully refundable prior to
the first day of the program. Registrants who do not attend and do not
cancel are subject to the complete fee. Participant substitutions may be
made with prior approval of the teaching staff.

----------------------------------------------------------------------------

Application Form: Fax to (203) 486-5005
Analytical Ultracentrifugation: Theory and Practice
March 19-21, 1996

Symposium March 22, 1996

National Analytical Ultracentrifugation Facility Biotechnology Center,
University of Connecticut, Storrs, Connecticut 06269-3149

Name:  _____________________________________

Name to put on badge:  _______________________

Social Security #:  ___________________________

Phone (day):  _______________________________

Organization:  _______________________________

Organization Address:_________________________

Street:  ____________________________________

City:  ______________________________________

Phone (evening):  ____________________________

Fax:_______________________________________


Registration fee: check one

     ____$1500       Workshop and Symposium

      ____$800        (not-for-profit organization)

      ____$200        March 22 Symposium only

 Method of Payment (once accepted)

     ____Bill me      ____Purchase Order enclosed.

Number:  ___________________________________


end application form

----------------------------------------------------------------------------

PROGRAM:

Review of basic theory and practice

   * sedimentation velocity
   * sedimentation equilibrium

Demonstration of basic experimental procedures using

   * Beckman Model E Analytical Centrifuge
   * Beckman XLA Analytical Centrifuge
   * optical absorption scanner method
   * Rayleigh interferometry
   * short and long column equilibrium sedimentation
   * velocity sedimentation

Demonstration of equilibrium techniques

Illustrations of experimental systems using proteins, glycoproteins, and
nucleic acids

   * simple proteins exhibiting ideal behavior
   * non-ideal behavior
   * interacting systems
   * heterogeneous systems

Data collection demonstrating

   * real-time interferometry
   * absorption scanning

Data analysis and interpretation

portable software furnished to participants for analytical procedures

LEARNING OBJECTIVES

Participants will:

   * be introduced to basic theory and practice of sedimentation velocity
     and equilibrium;
   * be shown how to design experiments to characterize macromolecular
     interactions;
   * use computer software for data analysis;
   * participate in small group discussions.

INSTRUCTORS:

Emory Braswell, Professor, Department of Molecular and Cell Biology,
University of Connecticut, Storrs, Connecticut.

Michael Johnson, Professor of Pharmacology and Internal Medicine,
University of Virginia Health Science Center,
Charlottesville, Virginia.

Jeffrey Lary, Research Associate, Department of Molecular and Cell Biology,
University of Connecticut, Storrs, Connecticut.

Thomas Laue, Associate Professor, Department of Biochemistry,
University of New Hampshire, Durham, New Hampshire.

Todd Schuster, Professor, Department of Molecular and Cell Biology,
University of Connecticut, Storrs, Connecticut.

Steven Shire, Senior Scientist, Pharmaceutical Research and Development
Department,
Genentech, Inc., South San Francisco, California.

Walter Stafford, Senior Scientist, Analytical Ultracentrifugation Research
Laboratory,
Boston Biomedical Research Institute, Boston, Massachusetts.

John M. Toedt, Graduate Research Assistant, Department of Molecular and Cell
Biology,
University of Connecticut, Storrs, Connecticut.

Jia-wen Wu, Facility Engineer, Analytical Ultracentrifugation Facility,
University of Connecticut, Storrs, Connecticut.

David Yphantis, Professor, Department of Molecular and Cell Biology,
University of Connecticut, Storrs, Connecticut.

Dan Zhu, Facility Scientist, Analytical Ultracentrifugation Facility,
University of Connecticut, Storrs, Connecticut.

Symposium following the workshop, March 22,

Call for Symposium Speaker List after 1/15/1996