From: Allen P. Minton <minton@helix.nih.gov>
  To  : RASMB@bbri.harvard.edu
  Date: Wed, 29 Nov 1995 10:20:10 -0500 (EST)

Differential sedimentation velocity

An investigator here at the NIH has mentioned that he would like to be
able to measure relatively small conformational changes associated with
Ca++ binding of a protein he is working on.  I mentioned to him that
changes of only a few percent in the sedimentation coefficient, presumably
reflecting relatively minor conformational changes, have been measured via
differential sedimentation velocity, but I have no personal experience
with this method. 

I would appreciate being contacted by someone who has done differential
sedimentation velocity experiments, preferably with the XLA, as I have
questions regarding this technique. 

Thanks -- Allen Minton