From: Jack Correia <jcorreia@umsmed.edu>
  To  : rasmb@bbri.eri.harvard.edu
  Date: Tue, 25 Jul 1995 13:43:50 -0500

Re: finding the right cells...

John,

     1) Has Beckman every made an external loader!  My understanding is they 
all come from Dave Yphantis' lab, or Dave's students, either at Storrs or 
the originals from NY.  I think Raymond Kikas made a lot of them.

     2) The reference for this cell is Anal Biochem 34, 237-261 (1970) by 
Ansevin, Roark and Yphantis.  [This expresses my dissatisfaction with XLA 
reference lists that do not reference the proper literature under the 
asumption that the "old" literature is out of data.]  The specifications are 
in the paper and can be made by an good machinist.

     3)  If my first point is correct then I might ask what other workers in 
fact do to alleviate this problem.  Overspeeding to pellet is not always 
possible, for example when you spin at 48K with small peptides or proteins 
to begin with.  It is my impression that many people actually use double 
sector cells for equilibrium runs, and not 6 channel centerpieces, thus 
limiting thenselves to three solutions instead of nine.

     4) Finally, {sorry Emory} I believe the National Center in Storrs can 
make external loaders for a price, although this may not be the long term 
solution!  

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| Dr. Jack Correia              |
| Univ. of Miss Medical Center  | 
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