From: Walt Stafford <STAFFORD@BBRI.ERI.HARVARD.EDU>
  To  : rasmb@BBRI.ERI.HARVARD.EDU
  Date: Fri, 10 Feb 1995 19:16:03 -0500 (EST)

Non Aqueous Sed. Equil.

From:	SMTP%"david@SPIN6.MCB.UCONN.EDU" 10-FEB-1995 17:08:16.73
To:	STAFFORD
CC:	
Subj:	Re: Non Aqueous Sed Eqil.

Date: Fri, 10 Feb 1995 16:59:22 -0500
From: david@SPIN6.MCB.UCONN.EDU
To: STAFFORD@BBRI.ERI.HARVARD.EDU
Subject: Re: Non Aqueous Sed Eqil.
X-VMS-To: SMTP%"STAFFORD@BBRI.ERI.HARVARD.EDU"
X-VMS-Cc: DAVID

We have used a number of nonaqueous solvents for sedimentation experiments.
Most pertinent, perhaps, are sedimentation equilibrium experiments that
do not develop much hydrostatic pressure to induce leakage. A reference
to such equilibrium experiments (both in short and long [~3mm] columns)  using
dichloroacetic acid as solvent is:

M Goodman, EE Schmitt and DA Yphantis, J. Amer. Chem. Soc. 84: 1283-88 (1962).

We have seen no problems in numerous velocity experiments using ethanol as
solvent. 

David Yphantis