From: NEEDHAM GREGORY F <NEEDHAM_GREGORY_F@Lilly.com>
  To  : RASMB@BBRI.ERI.harvard.edu
  Date: Fri, 03 Feb 1995 20:41:53 +0000 (GMT)

Non-aqueous Equilibrium sedimentation question

Non-aqueous Equilibrium sedimentation question
Has anyone ever done an equilibrium sed experiment in non-aqueous solvents?
e.g. TFE/buffer mixtures.  I assume the determination of partial specific
volume could be difficult, but I am questioning the integrity of the cells as
they interact with the organics.  I don't want TFE leaking from the cell and
getting inside the XLA.
Any thoughts or experience?
Greg Needham
gfn@lilly.com