From: Walt Stafford <STAFFORD@BBRI.ERI.HARVARD.EDU>
  To  : 
  Date: LESSOPEN=|lesspipe.sh %s

ANNOUNCEMENT of Univ. of Conn. AU WORKSHOP

ANALYTICAL ULTRACENTRIFUGATION: 
Theory and Practice 

WORKSHOP March 22-24, 1995

National Analytical Ultracentrifugation Facility
Biotechnology Center, University of Connecticut, Storrs, Connecticut, USA


* 	Interact with national experts in analytical ultracentrifugation.
* 	Learn characterization of recombinant produced proteins
* 	Work with hardware and software developed on -site at the 
	National Analytical Ultracentrifugation Facility.
* 	Receive individual attention to you research interests.
* 	See the Beckman XLA Analytical Centrifuge in Operation
* 	See real time interferometry in operation on Model E analytical
	ultracentrifuges.
* 	Learn about new specialized centrifuge cells.


INTENT and DESCRIPTION

Analytical Ultracentrifugation (AU) is a powerful method for the determination 
of absolute molecular weights and the study of self- association and interactions 
between biological molecules in solution. The combination of new computerized 
instrumentation, more rapid experimental protocols, and powerful data analysis 
techniques have led to a resurgence of interest in AU, particularly in 
biotechnology and pharmaceutical research.

The AU Facility in the Biotechnology Center was established in 1988 by grants 
from the National Science Foundation and the Connecticut Department of Higher 
Education. Creation of the facility follows the fundamental contributions of its 
staff to the development of AU to a wide range of basic and applied problems for 
over a quarter century. The staffed facility houses both Beckman Model E and 
XLA analytical centrifuges with their associated equipment. The facility is 
available to investigators for basic research through facility scientists or by 
collaboration with one of its principal investigators. Research projects from 
industry are encouraged.

The Analytical Ultracentrifugation Facility is pleased to offer an AU workshop 
designed for professionals with some previous knowledge of AU including:

 *  Industrial Scientists

 *  Academic Scientists

 *  Research Technicians

 *  Students

 The workshop will provide a conceptual framework and hands-on experience in 
modern computer-assisted data acquisition and data analysis for molecular weight 
determination, for study of molecular association- dissociation reactions and for 
determination of attendant equilibrium constants, with applications to proteins, 
glycoproteins and nucleic acids. One session will be devoted to development of 
experimental strategies for designing various kinds of molecular characterization 
problems.



Sponsored by the UConn Biotechnology Center, Beckman Instruments, Inc., 
UConn Department of Molecular & Cell Biology, Digital Equipment 
Corporation, Genentech, Inc., National Science Foundation, Perkin Elmer, 
Pfizer, Inc., SmithKline Beecham Pharmaceuticals, and others to be announced at 
the workshop.

SPECIAL PROGRAM FEATURES:

  - Limited workshop size for maximum interaction

  - Interactive lecture sessions

  - Data analysis software furnished to all participants


The registration fee of $1200 (or $600 for registrants of not-for- profit 
organizations) includes all costs of instruction, materials, software, parking, 
refreshments, lunches and reception. The fee does not include hotel 
accommodations.

Application is necessary. Faxed (203-486-5005) applications are encouraged. A 
letter of acceptance will include information on housing, transportation and an 
invoice for the registration fee, as appropriate.

For Information on:

*  Course content: call Todd Schuster at 203-486 4333

*  Payments and course logistics: call the Biotechnology Center at 203486-5011

*  Fellowships and Applications: call 203-486-5011

*  E-mail: Biotctrl@connvm.uconn.edu



Some registration fellowships are available to qualified graduate and post-
doctoral students. Call or send for a fellowship application form.

Refunds and Cancellations The registration fee is fully refundable prior to the 
first day of the program. Registrants who do not attend and do not cancel are 
subject to the complete fee. Participant substitutions may be made with prior 
approval of the teaching staff.



-------------------------------------- cut here X------------------------------------------

Application Form: Fax to (203) 486-5005 Analytical Ultracentrifugation: Theory 
and Practice   March 22-24, 1995

National Analytical Ultracentrifugation Facility Biotechnology Center, University 
of Connecticut, Storrs, Connecticut 06269-3149

 Name_____________________________________

Name to put on badge_______________________

Social Security #___________________

Phone (day)_______________________

Organization_____________________________

Organization Address:______________________

Street____________________________________

City______________________________________

Phone (evening)___________________________

Fax:_____________________________________


Registration fee: check one      __$1200      __$600 (not-for-profit organization)

 Method of Payment (once accepted)

     ___Bill me      ___Purchase Order enclosed.         
Number________________


----------------------------------- end application form ---------------------------------

PROGRAM:

Review of basic theory and practice

---  sedimentation velocity 
---  sedimentation equilibrium

 Demonstration of basic experimental procedures using

---  Beckman Model E Analytical Centrifuge 
---  Beckman XLA Analytical Centrifuge 
---  optical absorption scanner method 
---  Rayleigh interferometry 
---  short and long column equilibrium sedimentation

 Demonstration of equilibrium techniques

 Illustrations of experimental systems using proteins, glycoproteins, and nucleic 
acids

---  simple proteins exhibiting ideal behavior  
---  non-ideal behavior
---  interacting systems
---  heterogeneous systems

 Data collection demonstrating

--- real-time interferometry 
--- absorption scanning

 Data analysis and interpretation

- portable software furnished to participants  for analytical procedures


LEARNING OBJECTIVES

 Participants will:

*  be introduced to basic theory and practice of sedimentation velocity and 
equilibrium;

*  be shown how to design experiments to characterize macromolecular 
interactions;

*  use computer software for data analysis;

*  participate in small group discussions.

INSTRUCTORS:
Emory Braswell, Professor, Department of Molecular and Cell Biology, 
University of Connecticut, Storrs, Connecticut.

Jeffrey Lary, Research Associate, Department of Molecular and Cell Biology, 
University of Connecticut, Storrs, Connecticut.

Thomas Laue, Associate Professor, Department of Biochemistry, University of 
New Hampshire, Durham, New Hampshire.

Todd Schuster, Professor, Department of Molecular and Cell Biology, University 
of Connecticut, Storrs, Connecticut.

Steven Shire, Senior Scientist, Pharmaceutical Research and Development 
Department, Genentech, Inc., South San Francisco, California.

Walter Stafford, Principal Scientist, Department of Muscle Research, Boston 
Biomedical Institute, Boston, Massachusetts.

John M. Toedt, Graduate Research Assistant, Department of Molecular and Cell 
Biology, University of Connecticut, Storrs, Connecticut.

Jia-wen Wu, Facility Engineer, Analytical Ultracentrifugation Facility, 
University of Connecticut, Storrs, Connecticut.

David Yphantis, Professor, Department of Molecular and Cell Biology, 
University of Connecticut, Storrs, Connecticut.

Dan Zhu, Facility Scientist, Analytical Ultracentrifugation Facility, University of 
Connecticut, Storrs, Connecticut.

 Guest speakers at some evening sessions.