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From: Geoff=Howlett%BioChem%UNIMELB@muwaye.unimelb.EDU.AU
To : rasmb@bbri.eri.harvard.edu
Date: Wed, 11 May 94 12:27:57 +1000
...no subject...
Ok, so I don't mind being the fall guy. I have a few questions concerning
recent messages:
1. What is the Van Holde-Weischet method and what advantages does it offer?
2. How big are these sloping baselines and are they machine or sample
dependent? i.e. is it a 10% slope from meniscus to base and is it only
apparent with a full cell? Is there a sample (say bromphenol blue) which
everyone has access to which would allow sloping baselines to be checked on
different machines? For my part I am pretty happy with my baselines.
3. I agree with the comment that Beckman have to be commended for the
magnificent machine they have produced. It is still mind-boggling to me that
such accurate and reproducible data can be obtained with a sample spinning at
60,000 rpm. Commenting on possible improvements to the software and machine
design is a positive step which I would think would be viewed favourably by
potential customers and can only lead to a better machine.
Would it make sense to start with the software and make a list of some of the
limitations? I keep anticipating a new package will be developed. Is there
a projected release date, what are the improvements and is it too late to
have an input into the new version?
Geoff Howlett
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