Figure 1. Plot of concentration versus radius
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Figure 2. Plot of time derivative vs radius
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Pairs of curves are subtracted at corresponding radial positionsto produce the baseline corrected time derivative pattern.
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Figure 3. Plot of averaged time derivative versus s*
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The x-axis for each of the curves from Figure 2 was transformed from radius to s* by the following relationship to compensate for the different times of sedimentation: This transforms the x-axis from the stationary reference frame of the ultracentrifuge cell to the moving reference frame of sedimentation.
where w is the angular velocity of the rotor
t is the sedimentation time
r is the radius, and
rm is the radius of the meniscus.
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